Journal: Translational Oncology

Article Title: The expression of Slit2 and Robo1 increased during retinoic acid syndrome in acute promyelocytic leukemia and impacted differentiated cell migration

doi: 10.1016/j.tranon.2022.101370

Figure Lengend Snippet: The characteristics of the 62 cases of APL.

Article Snippet: Slit2 Quantitative ELISA kit (E-EL-H0931c, Elabscience, China), IL-8 ELISA kit (#Q8000B, R&D, USA), and IL-1β ELISA kit (#DLB50, R&D, USA) were used to measure the levels of Slit2, IL-8, and IL-1β, respectively. rhIL-8 (200–08 M, Peprotech, USA) and rhSlit2-N (150-11, Peprotech, USA) were used to perform the migration assay using transwell.

Techniques:

Journal: Translational Oncology

Article Title: The expression of Slit2 and Robo1 increased during retinoic acid syndrome in acute promyelocytic leukemia and impacted differentiated cell migration

doi: 10.1016/j.tranon.2022.101370

Figure Lengend Snippet: (A) Results of Robo1 in differentiated cells by immunofluorescence. 1–2, from RAS+ patients; 3, from RAS- patients; and 4, from the control individual. (B) Transwell assay results of NB4 cells as rhIL-8 and Slit2-N were added to the lower layer. 1–1, 2–1, and 3–1 showed the NB4 cells in the upper layer after migration; 1–2, 2–2, and 3–2 showed the migrated NB4 cells in the lower layer; 1–2 showed the FBS in the lower layer, 2–2 showed the addition of Slit-N; 3–2 showed the addition of rhIL-8 and Slit2-N. The migration frequency increased as rhIL-8 and Slit2-N were added to the lower layer. (C) Robo1 protein estimation using western blotting: line 1, when RAS developed in a patient; line 2, before the development of RAS; line 3, after the development of RAS; line 4, in an APL patient without RAS; line 5, in a control; line 6, in a mild-RAS patient; line 7, in a severe-RAS patient. (D) Transwell assay results of co-culture of NB4 and A549 cells with rhIL-8 and Slit2-N. 1–1, 2–1, and 3–1 show the rest NB4 cells in the upper layer after migration; 1–2, 2–2, and 3–2 show the migrated NB4 cells and A549 cells in the lower layer; 2–2 shows the addition of rhIL-8; 3–2 shows the addition of rhIL-8 and Slit2-N. The migration frequency increased as rhIL-8 and Slit2-N were added to the lower layer.

Article Snippet: Slit2 Quantitative ELISA kit (E-EL-H0931c, Elabscience, China), IL-8 ELISA kit (#Q8000B, R&D, USA), and IL-1β ELISA kit (#DLB50, R&D, USA) were used to measure the levels of Slit2, IL-8, and IL-1β, respectively. rhIL-8 (200–08 M, Peprotech, USA) and rhSlit2-N (150-11, Peprotech, USA) were used to perform the migration assay using transwell.

Techniques: Immunofluorescence, Control, Transwell Assay, Migration, Western Blot, Co-Culture Assay

Journal: Translational Oncology

Article Title: The expression of Slit2 and Robo1 increased during retinoic acid syndrome in acute promyelocytic leukemia and impacted differentiated cell migration

doi: 10.1016/j.tranon.2022.101370

Figure Lengend Snippet: The differences in Slit2, Robo1, and cytokines between RAS+ and other groups.

Article Snippet: Slit2 Quantitative ELISA kit (E-EL-H0931c, Elabscience, China), IL-8 ELISA kit (#Q8000B, R&D, USA), and IL-1β ELISA kit (#DLB50, R&D, USA) were used to measure the levels of Slit2, IL-8, and IL-1β, respectively. rhIL-8 (200–08 M, Peprotech, USA) and rhSlit2-N (150-11, Peprotech, USA) were used to perform the migration assay using transwell.

Techniques: Control, Enzyme-linked Immunosorbent Assay

Journal: Translational Oncology

Article Title: The expression of Slit2 and Robo1 increased during retinoic acid syndrome in acute promyelocytic leukemia and impacted differentiated cell migration

doi: 10.1016/j.tranon.2022.101370

Figure Lengend Snippet: The correlation of Slit2, Robo1, and cytokines with the development of RAS in 16 patients.

Article Snippet: Slit2 Quantitative ELISA kit (E-EL-H0931c, Elabscience, China), IL-8 ELISA kit (#Q8000B, R&D, USA), and IL-1β ELISA kit (#DLB50, R&D, USA) were used to measure the levels of Slit2, IL-8, and IL-1β, respectively. rhIL-8 (200–08 M, Peprotech, USA) and rhSlit2-N (150-11, Peprotech, USA) were used to perform the migration assay using transwell.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Translational Oncology

Article Title: The expression of Slit2 and Robo1 increased during retinoic acid syndrome in acute promyelocytic leukemia and impacted differentiated cell migration

doi: 10.1016/j.tranon.2022.101370

Figure Lengend Snippet: Correlation between the expressions of Slit2, Robo1, and IL-8 and the severity of RAS.

Article Snippet: Slit2 Quantitative ELISA kit (E-EL-H0931c, Elabscience, China), IL-8 ELISA kit (#Q8000B, R&D, USA), and IL-1β ELISA kit (#DLB50, R&D, USA) were used to measure the levels of Slit2, IL-8, and IL-1β, respectively. rhIL-8 (200–08 M, Peprotech, USA) and rhSlit2-N (150-11, Peprotech, USA) were used to perform the migration assay using transwell.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Translational Oncology

Article Title: The expression of Slit2 and Robo1 increased during retinoic acid syndrome in acute promyelocytic leukemia and impacted differentiated cell migration

doi: 10.1016/j.tranon.2022.101370

Figure Lengend Snippet: (A) Robo1 expression measured using FCM in 16 APL patients with RAS from day 1 to day 21, showing the development of RAS from 9% to 30%. (B-C) The gene expression of CXCR1, CXCR2, CXCR3, CXCR4, Robo1, Slit2, and cytokines in differentiated cells of the 16 APL patients with RAS. CXCR1, CXCR2, CXCR3, and CXCR4 showed no relation to RAS. The expression of IL-8, IL-1β, and Robo1 upregulated when RAS developed. (D) Robo1, IL-8, and IL-1β in different groups, results showed they were higher in the RAS+ group than the RAS- and control groups.

Article Snippet: Slit2 Quantitative ELISA kit (E-EL-H0931c, Elabscience, China), IL-8 ELISA kit (#Q8000B, R&D, USA), and IL-1β ELISA kit (#DLB50, R&D, USA) were used to measure the levels of Slit2, IL-8, and IL-1β, respectively. rhIL-8 (200–08 M, Peprotech, USA) and rhSlit2-N (150-11, Peprotech, USA) were used to perform the migration assay using transwell.

Techniques: Expressing, Control